Clinical and Morphological Analysis of Efficacy of Intraperitoneal Ozone Application in Experimental Colitis: Preclinical Randomized Experimental Study

Background. Inflammatory bowel diseases — Crohn’s disease and ulcerative colitis are chronic gastrointestinal affecting young people of working age. An alternative to basic therapy (5-aminosalicylic acid) for inflammatory is the use ozone, which has anti-inflammatory, immunomodulatory, antibacterial properties no side effects in therapeutic concentrations. Objective. To perform clinical morphological analysis efficacy intraperitoneal ozone application experimental colitis. Methods. The study was conducted on 73 male Wistar rats weighing 200-250 g. animals were divided into four groups by simple randomization. Check studies performed second, fourth sixth days. Oxazolone-induced simulated two stages using a 3%-alcohol oxazolone solution. Ozone-acid mixture obtained “UOTA-60-01” unit (“Medozone”, Russia). Rectal suppositories with 5-aminosalicylic acid prepared basis rectal “Salofalk”. Clinical status assessed daily according activity index (DAI) scale. Morphological evaluation colon lesion tissue fragments carried out PrimoStar microscope (CarlZeiss, Germany). Colon damage (TDI). Statistical SPSS Statistics 19 (IBM, USA). Results. picture large intestine oxazolone-induced days 2, 4 6 consistent changes typical humans. Daily insufflation at dose 0.05 mg/kg leads partial restoration DAI, reduction neutrophils, eosinophils, histiocytes, fibroblasts lesion, as well decrease ulcerous defect diameter TDI. insufflations compared 50 mg every 12 hours less marked DAI day 4; number plasma cells, histiocytes 6; lymphocytes 6. Conclusion. ozone-induced correlates positive effect driven its anti-inflammatory through activation Nrf2 antioxidant inhibition Keap1.